CpxA (N-Term) antibody

Catalog No. ABIN1720869

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB. Suitable for Yersinia pseudotuberculosis.

Quick Overview for CpxA (N-Term) antibody (ABIN1720869)

Target: CpxA

Reactivity: Yersinia pseudotuberculosis

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Yersinia pestis, Yersinia enterocolica and related species

Purification: serum

Immunogen: Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. The antigen originates from enteropathogenic Yersinia pseudotuberculosis YPIII, B1JQV1, NCBI annotated locus tag YPK_4133.

Application Details

Application Notes: 1 : 2000 with standard ECL (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Handling Advice: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details

Target: CpxA

Background: CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.

Molecular Weight: expected: 51.8 kDa, appearent: 52-53 kDa